Fig. 2. Effects of TH gene overexpression or silence in ankle joints of mice with CIA on Th17 and Treg cell function. 2×10⁵ TU of TH lentiviral vector or TH-shRNA lentiviral vector was injected into ankle joints of CIA mice on day 33 post-immunization. Levels of ROR-γt and Foxp3 protein expression (A) and IL-17, IL-22, IL-10 and TGF-β gene expression (B) as well as contents of IL-17, IL-22, IL-10 and TGF-β1 (C) in ankle joints were measured on day 69 post-immunization. ^**p<0.01, versus intact control; ⁺⁺p<0.01, compared with TH mock control; ^#p<0.05, ^##p<0.01, compared with Scr-shRNA control.